BD CD3抗體（PE-CY7標記）
BD CD3抗體（PE-CY7標記）557851
The SK7 (Leu-4) monoclonal antibody specifically binds to the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). The CD3 antigen is present on 61% to 85% of normal peripheral blood lymphocytes 60% to 85% of thymocytes and on Purkinje cells in cerebellum. The soluble form of this antibody has a mitogenic effect on most peripheral blood T lymphocytes, provided appropriate functional monocytes are present.
BD CD3抗體（PE-CY7標記）557851
Brand
BD Pharmingen™
Alternative Name
CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Vol. Per Test
5 µl
Isotype
Mouse BALB/c IgG1, κ
Reactivity
Human (QC Testing)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human Thymocytes
Workshop No.
II T118; III T492
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
557851
Format
PE-Cy™7
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
785 nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.
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