午夜小网站 I 91热热热 I 少妇人妻挤奶水中文视频毛片 I 国精产品99永久一区一区 I 欧美黑人巨大videos极品 I 丰满老女人乱妇dvd在线播放 I 精品一区二区在线看 I 精品亚洲国产成人av I 在线va无卡无码高清 I 亚洲精品大全 I av天天射 I 国产无遮挡无码视频在线观看 I 少妇久久久久久久久久 I 久久精品亚洲精品 I 成人视屏在线 I 午夜色区 I 丁香七月激情 I 亚洲风情av I 97色爽 I 亚洲黄视频在线观看 I 在线播放免费人成毛片乱码 I 青草av久久一区二区三区 I 日本免费视频在线观看 I 久久伊人在 I 成人精品一区二区三区中文字幕 I 2018av天堂在线视频精品观看 I 日韩经典中文字幕 I 亚洲麻豆国产 I 欧洲国产精品无码专区影院 I 一级a毛片高清视频 I 碰超在线97人人 I 看欧美日韩国产 I 小泽玛丽亚在线观看 I 成人在线高清免费 I 精品国产网站

fermentas逆轉錄試劑盒RevertAid First Strand cDNA Synthesis Kit說明書

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit是一套用于以RNA為模板高效合成*鏈cDNA的完整系統，可合成長達13kb的cDNA。試劑盒配套提供的RiboLockRNase Inhibitor，可有效防止RNA模板的降解。試劑盒配套提供oligo(dT)18引物和隨機六聚體引物。oligo(dT)18引物可以選擇性地與mRNA中的poly (A)尾結合。隨機六聚體引物無需poly (A)尾，因此可轉錄mRNA 5’-端區域，也可以無poly (A)尾的RNA (如micoRNA)為模板合成cDNA。該試劑盒也可使用基因特異性引物。

優點：

• 合成全長的*鏈cDNA，zui長可合成13kb的cDNA
• *反應溫度為42℃
• *一提供RT反應所需的所有組分

應用:

• *鏈cDNA合成，作為RT-PCR和實時RT-qPCR的模板
• 構建全長cDNA文庫
• 反義RNA合成

fermentas逆轉錄試劑盒RevertAid First Strand cDNA Synthesis Kit 

RevertAid First Strand cDNA Synthesis Kit
Features
·         Full-length first strand cDNA up to 13 kb.
·         Increased reaction temperatures in the range of 42-50°C.
·         Supplied with the recombinant RiboLock? RNase Inhibitor.
·         Complete – oligo(dT)18 and random hexamer primers included with the kit.
Applications
·         First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2).
·         Construction of full length cDNA libraries.
·         Generation of probes for hybridization.
·         aRNA synthesis.
Description
The RevertAid? First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb.
The kit uses RevertAid? Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase.
The recombinant RiboLock? RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C.
The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits.
The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol). 
Quality Control
The kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.

is Kit

RevertAid First Strand cDNA Synthesis Kit合成全長cDNA
1 μg小鼠心臟總RNA作為模板，用oligo(dT)18 引物進行逆轉錄反應。由此合成的cDNA，再作為Taq DNA Polymerase后續PCR擴增的模板。末端2024 bp片段的成功擴增，表明長度為13.8 kb的全長RNA片段得到成功的逆轉錄。

產品組成
RevertAid First Strand cDNA Synthesis Kit包含RevertAid Reverse Transcriptase、RiboLock RNase Inhibitor、5x Reaction Buffer、dNTP Mix、Oligo(dT)18 引物、隨機六聚體引物、對照GAPDH RNA、10 μM 正向GAPDH引物、10 μM反向GAPDH 引物以及nuclease-free water。